A sustainable data processing approach using ultraviolet-spectroscopy as a powerful spectral resolution tool for simultaneously estimating newly approved eye solution in the presence of extremely carcinogenic impurity aided with various greenness and whiteness assessment perspectives: Application to aqueous humor

Drug impurities are now seen as a major threat to drug safety around the world, especially when it comes carcinogenic impurities. Here, we present first spectrophotometric approach for quantification of lignocaine and fluorescein in presence 2,6-xylidine, lignocaine’s impurity. The depends on overcoming unresolved bands through data processing strategy employing 10 affordable, simple, sensitive methods. Fluorescein analysis (1–16 μg mL −1 ) was performed using direct ultraviolet spectrophotometry (D 0 at 478 nm; then, ratio subtraction method allowed removal interference caused by spectrum. From resulting spectra, 2,6-xylidine (40–160 can be efficiently determined 280 nm. However, (72–320 analyzed different ultraviolet-based methods, including continuous wavelet transform, derivative Savitzky–Golay filters, mean centering, second spectra difference spectrophotometry, extended subtraction, absorbance Q-absorbance ratio, area under curve. In line with International Council Harmonization Technical Requirements Pharmaceuticals Human Use (ICH), presented evaluated its coefficients determination, detection limits, relative standard deviations. Similarly, developed assessed whiteness, greenness, sustainability five assessment tools, National Environmental Method Index, Eco-Scale Assessment, Complementary Green Analytical Procedure Greenness Metric, RGB12, offering promising results. Owing satisfactory analytical performance, besides sustainability, affordability, simplicity, cost efficiency their application quality control situ minimal-infrastructure laboratories increases, increasing surveillance potential.